The utility model discloses a biochip reaction unit, including blank board, biochip, cover glass, bio -probes, slide glass, inclined plane, recess, glass piece, objective table and arc recess, the objective table is installed all around to blank board, the objective table is equipped with the inclined plane, the inclined plane top is equipped with the recess and is stamped the glass piece, placed on the objective table biochip biochip top -down is by cover glass, bio -probes, slide glass triplex, and the biochip lower end is equipped with the arc recess, and the device passes through on the inclined plane combination of glass piece and recess, and environment inclosed problem but also prevented that reagent from overflowing biochip has inadequately increased the effective volume of reaction among the solve biochip reaction sequence, simultaneously because this device contains the four objective table, consequently can the simultaneous reaction in the multisample control experiment, and ageing strong, with distinct contrast, the biochip setting -in is gone into the objective table and is made the biochip will not moved easily in addition.