The utility model discloses a micro -fluidic box autoanalysis biochip, the last hot chaining pin of multiunit that is provided with of chip body, the left side on the chip body is provided to first reagent room, second reagent room, third reagent room, fourth reagent room and fifth reagent room, first reagent room, second reagent room, third reagent room, fourth reagent room, fifth reagent room are connected with the reacting chamber through one -way pump and transport microtubule respectively, the right side on the chip body is provided to reacting chamber and waste liquid room, the reacting chamber is connected with the waste liquid room, be equipped with the executor between first reagent room, second reagent room, third reagent room, fourth reagent room, fifth reagent room and the one -way pump, a check valve is provided on the transport microtubule between one -way pump and the reacting chamber. Analysis, the effectual efficiency of sample liquid analysis and the accuracy of analysis of having improved can be processd to multiple sample liquid to the biological core of the micro -fluidic box autoanalysis.