The utility model relates to a microbial inoculum inoculation device belongs to microbial manure inoculation technical field, microbial inoculum inoculation device mainly include: plank frame, plastic bag and the 338 holes dish of growing seedlings, 338 holes grow seedlings set, plastic bag and plank frame match each other, plank frame connect to form through the draw -in groove by first plank and second plank, first plank, second plank broadside are provided with the cutting ferrule, plank rest base portion is fixed by the mount, be equipped with the stationary blade on the mount, each edge of plastic bag be provided with and be used for the card strip that seals, the card strip is corresponding with the cutting ferrule, these microbial inoculum inoculation device materials are simple, in the use, the design of cutting ferrule can avoid the microbial inoculum to run off on the plank frame plastic bag fastening, the design of mount and stationary blade, can guarantee the microbial inoculum in the plastic bag liquid level level and also can move the plank frame under the condition of not taking out the plastic bag, the design of first through -hole and second through -hole makes the abandonment microbial inoculum discharge easily.