The utility model discloses a crooked fungus ELISA kit of jejunum, comprises a box bod, be equipped with valve bag and liner in the box body, it regards as the crooked fungus monoclonal antibody's of anti jejunum of seizure antibody enzyme to mark the reaction plate to be equipped with the peridium in the valve bag, be equipped with several container hole on the liner, the container is downthehole to be equipped with positive control solution pipe, negative control solution pipe, 10 concentrated cleaning solution pipes, color development liquid pipe respectively, terminate the liquid pipe, weak solution pipe and detected the crooked fungus monoclonal antibody solution pipe of anti jejunum of antibody by the conduct of horseradish peroxidase mark. The utility model discloses a kit requires to hang down to the pretreatment of sample, and is easy and simple to handle, has the higher sensitivity and the degree of accuracy, and detection limit is 105cfuml, and the specificity is good with stability to it does not all have cross reaction to eat source nature pathogenic bacteria with great majority.