The utility model discloses a former liver histocyte piece -rate system that takes place of, by control panel, circulating pump pipeline, cell separation box and cell enrichment bottle are constituteed, and control panel can start operating system, monitored control system operational environment, the circulating pump pipeline pours into the cell parting liquid that contains the collagenase in the liquid storage pot into hepatic tissue, provides to last dabbling steady pressure, cell separation box guarantees the hepatic tissue and accept the continuously effect of filling of the cell parting liquid that roll extrusion formula pump goes into in sealing aseptic environment, and the cell separates gradually, and the screen cloth that loops through the different apertures of three -layer along with the cell parting liquid progressively separates impurity and manadesma, isolates the primary cells suspension at last, formerly takes the place of single cell suspension to flow in cell enrichment bottle, finally realize liver tissue in primary cells separate the enrichment. The utility model discloses system design is reasonable, and whole disengaging process seals asepticly, and control is accurate, contains the perfusate ability cyclic utilization of collagenase, reduce cost, convenient and practical.