The utility model provides a motionless tubular column injection test of three -layer water injection well and test tubular column that seals, motionless tubular column injection test of three -layer water injection well and test tubular column that seals include Y531 type packer, constant pressure switch and the ZY521 type packer that connects gradually from the top down through oil pipe. Through setting up Y531 type packer and ZY521 type packer, can seal the operation that the seperated layer water injection was realized to ZY521 type packer and Y531 type packer through seat in proper order, reach the purpose of testing a job of the injection test of accomplishing the oil reservoir under the condition of motionless tubular column and packer.