The utility model relates to a show technical field, disclose a mould and light guide plate and manufacturing method, equipment, display module assembly. The mould of preparation light guide plate includes that at least two are annotated the runner to the runners of annotating advance gluey mode preparation light guide plate more, can make thinner light guide plate. The mould still includes at least one drainage mouth, the drainage mouth corresponds different bursts of interfaces of expecting that stream joins and sets up, and the melting material that joins interface department is extrudeed the drainage mouth that corresponds, and the follow the drainage mouth is discharged for different strands of material flow join the integration that interface department can be fine, eliminate the weld mark, guarantee the optical property of light guide plate. Consequently, through the technical scheme of the utility model thinner light guide plate can be makeed, the optical property of light guide plate can be guaranteed moreover.