The utility model discloses a grass family plant leaf epidermal cell blotter relates to a device that separates plant leaf epidermal cell trace. The device includes slide glass, buckle, and the rubber ring layer that sets up of buckle, forms rubber board cavity between rubber ring layer and the buckle, and the another side of buckle is provided with the buckle part of bleeding. Rubber ring layer in the the device structure plays the cushioning effect of protection slide glass when receiving the extrusion, reaches to prevent that the slide glass is broken, the rubber ring layer can also be bled when the buckle and hold the slide glass when part is bled as the gasket of slide glass, when using, drips glue and scribbles on the slide glass, utilizes the device to clip grass family plant leaf, and complete the peeling off of the upper and lower epidermis of blade when the slide glass is opened reaches and do benefit to the complete trace of blade epidermis cell morphology characteristic on the observation slide glass.