The utility model belongs to the laboratory glassware field specificly provides a volumetric flask cleaning brush. A volumetric flask cleaning brush, brush hair on includes handle, brush -holder stud and locates the brush -holder stud, the brush -holder stud includes that main brush -holder stud, first minute brush -holder stud and second divide the brush -holder stud, first, second divides brush -holder stud one end all to articulate with main brush -holder stud is terminal, and first, two fens brush -holder studs are equipped with the spring within a definite time. When the thin bottleneck that passes the volumetric flask when the spring gets into the great hypomere of opening, the spring extension, first, the second that links to each other with the spring divides the brush -holder stud the reverse bottle wall of opening and pressing close to the volumetric flask, it both can clean the volumetric flask to rotate the handle. The utility model discloses simple structure, it is clean light, laborsaving, efficient.