Experimental device for methanogen degradation coal seam is remained guanidine glue and is had product gas concurrently is including temperature control unit, biological methane metabolism environment monitor cell, reservoir injury evaluation unit and product gas evaluation unit, temperature control unit includes thermostated container and intelligent thermostatic control air conditioner, the thermostated container is the open box in top, and sealing connection can be dismantled in the thermostated container top has the sealed lid of box, and intelligent thermostatic control air conditioner installation is in thermostated container one side, biological methane metabolism environment monitor cell is located the thermostated container with product gas evaluation unit, biological methane metabolism environment monitor cell includes metabolism reaction vessel and rotational viscometer, the utility model discloses preparation simple process, compact structure, low cost, experimental performance height can verify that methanogen degradation guanidine produces gases such as methane when gluing, glue the application of fracturing fluid in the coal bed gas trade for the guanidine and provide the scientific basis.