The utility model provides a magnetism microballon fluorescence detection system, its characterized in that: including sample draw -out device, electro -magnet, magnetism microballon quantity monitoring devices and fluorescent label thing detection device, the sample draw -out device include imbibition syringe needle, quartz capsule and imbibition pump, the quartz capsule is connected to the imbibition syringe needle, and the imbibition pump is connected to the quartz capsule, fluorescent label thing detection device include exciting light light source, photoelectric converter no. 1, excite the light path and collect light path no. 1, magnetism microballon quantity monitoring devices including collecting light path no. 2 and photoelectric converter no. 2. Through the electro -magnet with magnetism microballon gathering at detection area, make the magnetism microballon improve thousands of times in detection area's concentration, the fluorescence intensity of fluorescent label thing then can improve up to ten thousand times, effectively subducts owing to wash not thoroughly the interference that brings the testing result.