The utility model provides a pair of sugarcane culture medium sterilization in bags holds device, include the culture medium support and hold the box, the culture medium support includes bottom, elastically returning portion, presss from both sides the portion of closing of hugging closely, press from both sides the portion of closing of hugging closely under the effect of elastically returning portion return elasticity, the laminating together each other, bottom, elastically returning portion enclose into a culture medium and hold the room, hold the box including side wall and bottom surface, the shape of cross section of bottom surface is the even wave of a plurality of crest and trough constitution, the bottom and the trough bottom of side wall maintain an equal level, evenly are equipped with a plurality of bleeder vents on the trough. The utility model discloses simple structure guarantees culture medium support position fixed in holding the box, can not take place the danger that the culture medium support is emptyd and the culture medium is revealed, hold the wave and the bleeder vent setting of box bottom face, it can the multilayer stack to guarantee to hold the box, but the inner space of make full use of sterilization pot, and the culture medium is heated evenly when ensureing to sterilize simultaneously.