The utility model discloses a CIK cell culture kit, including box body, the lid that can open and the anti people CD3 monoclonal antibody blake bottle of peridium, from left to right set gradually in the box body and have or not blood serum medium standing groove, lymph parting liquid standing groove, blake bottle standing groove and cytokine memory cell, the anti people CD3 monoclonal antibody blake bottle of peridium is placed in the blake bottle standing groove, is cytokine memory cell cytokine reorganization people IL 2 the standing groove, cytokine reorganization people IL 2 the quantity of standing groove is two. The anti people CD3 monoclonal antibody's of this kit peridium blake bottle effectively improves the growth rate and the quantity of CIK cell, for tumour patient better adoptive immunity treatment provision method of acquisition in the short time.