The utility model relates to a device based on acetylene suppression method survey soil nitrification, the device is provided with outer PVC and cultivates the bucket, outer PVC adopts the rubber stopper to connect the three -way valve in the middle of cultivateing the bucket upper end, outer PVC trains and to be equipped with a plurality of PVC pipes that have an aperture with the edge in the bucket and to divide and train the bucket. The utility model discloses utilizing 10% concentration acetylene can restrain nitrification N2O and produce and to restore to N2 with denitrification in -process N2O, does the messenger nitrify anti - nitration result all exists with N2O, sets up the control experiment, and the latent gesture with anti - nitrification is nitrified in the estimation. Adopt acetylene restraining method, the result is accurate and the operation is more simple and convenient, and original state soil is cultivateed to open -air normal position, has kept the incubator intact sealed, the utility model discloses utilize PVC pipe fitting and end cap self -control incubator, and it is respond well.