The utility model relates to a poultry cultivation techniques field, concretely relates to breeding hens is seminal fluid heat preservation box for insemination, outer box body and interior box body, outer box body cladding in outside the interior box body, interior box body is hollow thin walled structure interior box body inside has a confined cavity, be provided with a plurality of through -holes on the epipleural of interior box body, separate between the adjacent through hole and arrange, place the heat preservation post that is used for holding the breeding hens seminal fluid in the through -hole, the heat preservation post opens for the upper end, lower extreme confined hollow tubular structure, the lateral wall of heat preservation post with be clearance fit between the through -hole, the upper end tip of heat preservation post is towards deviating from the protruding flange that forms in one side of heat preservation post, the overlap joint of flange is in on the interior box body epipleural, make the heat preservation post of flange below is located in the cavity of interior box body. The utility model provides a breeding hens is seminal fluid heat preservation box for insemination enough reduces ambient temperature and to the influence of breeding hens seminal fluid, guarantees breeding hens seminal fluid quality.