The utility model relates to a pretreatment of samples device of gene sequencing, including broken elution separator, flush fluid accumulator, eluant accumulator and nucleic acid accumulator, flush fluid accumulator, eluant accumulator, nucleic acid accumulator respectively with broken elution separator is connected, even there is the pivot broken elution separator bottom, the pivot even has the rotation motor, the inside nucleic acid filter membrane inner chamber that is equipped with of broken elution separator, the inside supersonic generator that is equipped with of broken elution separator, nucleic acid accumulator bottom is equipped with the nucleic acid spray gun, nucleic acid spray gun below is equipped with the nucleic acid extraction conveyer belt, place nucleic acid on the nucleic acid extraction conveyer belt and preserved the card. The utility model discloses a broken elution separator pair cell liquid is broken, wash, separate these processes integrates, carries out the pretreatment of samples process synthesis of gene sequencing in an equipment, has simplified the preliminary treatment process, respond well.