The utility model provides an online measurement system in succession of glass substrate stress belongs to the glass substrate stress testing technique field. The system includes and carries out the transport mechanism that conveys to glass substrate, carries out the upper portion centre gripping guiding mechanism and the lower part centre gripping guiding mechanism that lead to glass substrate, distributes at the stress detector of last lower part centre gripping guiding mechanism both sides, and the counter stress detector leads and driven actuating mechanism, actuating mechanism is two setsof, and the stress detector to both sides leads and drives respectively. Adopt the utility model discloses the system can realize the continuous measurement to glass substrate stress, can realize thescanning survey of the whole board stress of glass substrate, simultaneously also can fixed position continuous sweep, realize the key detection to strategic locations stress. The utility model discloses glass substrate stress measurement system detects glass substrate stress, can improve the ageing and continuity that glass substrate stress detected, has reduced bad loss, improves product quality control ability.