The embodiment of the utility model discloses multichannel fluorescence detection system. Multichannel fluorescence detection system includes: a n excitation light source is used for launching the light beam of different wave bands respectively, a n light bundle adjustment unit, each light bundle adjustment unit corresponds with an excitation light source for the accurate straightness and the direction of the light beam that adjustment excitation light source sent, first convergent mirror sets up between light bundle adjustment unit and need checking sample for the light beam convergence thatwill be bare the outgoing of bundle adjustment unit is to the need checking sample, and in order to arouse production fluorescence, the fluorescence that produces need checking sample carries out thecollimation, light -splitting unit is used for receiving the fluorescence behind the first convergent mirror collimation to carry out the beam split to fluorescence, tell the provocative fluorescenceof light beam that each excitation light source sent, and make fluorescence project corresponding fluorescence detection unit respectively, a n fluorescence detection unit, each fluorescence detectionunit corresponds with an excitation light source, and the fluorescence detection unit is used for detecting fluorescence. The embodiment of the utility model provides an accuracy and the detection efficiency of testing result have been improved.