The utility model discloses multifunctional plant tissue culture bottle bottle lid belongs to biological experiment device technical field, including bottle and bottle lid, to connect through screw Ibetween bottle and the bottle lid, the bottle lid includes upper cover and lower cover, and the upper cover middle part is equipped with downward open -ended recess, and the lower cover middle part isequipped with the cavity tubular column, is connected through screw II between cavity tubular column and the recess, and cavity tubular column and lower cover junction are equipped with the opening,cover the filter membrane on the opening covers under, and still is equipped with the bell mouth, is equipped with the toper stopple in the bell mouth. The utility model discloses multifunctional plant tissue culture bottle bottle lid, the cooperation of upper cover and lower cover is used, both can make in the blake bottle closedly totally, turns on the upper cover, also can realize blake bottleand external air exchange, in addition, uses through the cooperation of bell mouth and toper stopple, need not to open and to replenish sterile water or nutrient solution, has improved the additionalefficiency of culture medium, has also reduced the microbial contamination probability.