The utility model discloses a combined colon mirror biopsy specimen collection bottle which comprises a collection bottle body, and the collection bottle body is cylindrical and comprises a bottle body and a bottle cover in threaded connection with the bottle body. Wherein the separation frame is in the shape of a general outline of a colon of a human body and is sequentially provided with eight accommodating cavities according to the colon structure; The separation frame comprises an annular plate and a bent plate of a colon structure, seven vertically-arranged separation plates are arrangedbetween the annular plate and the bent plate, the separation frame can be divided into eight containing cavities, and a standby storage cavity is formed in the middle of the separation frame; And thepartition plate is inserted into open grooves formed in the annular plate and the bent plate. The utility model provides a combined colon mirror biopsy specimen collection bottle which is provided with a separation frame, can effectively prevent the problems that the number of sampled specimens is insufficient, and the specimens are not easy to identify and mark, and can save the workload and improve the integrity and effectiveness of the specimens and the accuracy of pathological examination.