The utility model relates to the field of cultivation devices, in particular to a layered cultivation device for miniature potted flowers of positive flowers and negative flowers. A layered cultivation device for micro potted flowers of positive flowers and negative flowers comprises a supporting and moving system (1), an upper-layer cultivation pipeline (2), a lower-layer cultivation pipeline (3), a water and fertilizer supply and recovery system (4) and a controller (5). The device is convenient to use, and layered cultivation and automatic irrigation operation of micro potted flowers of positive flowers and negative flowers can be achieved.