The utility model relates to the technical field of tobacco planting. The on-ridge positioning and punching device for tobacco transplanting comprises a machine frame, the punching machine comprises arack, walking wheels, a conical punching head, a lifting vertical rod, a rotating disc, a lifting sleeve plate and a lifting sleeve. The rotating disc is fixedly mounted at the front end of the rotating rod along the central axis; at least three installation holes are formed in the rotating disc at equal intervals, an installation bolt is arranged in one installation hole in a penetrating mode, the lifting sleeve plate is arranged on the installation bolt in a sliding and sleeving mode through a strip-shaped through hole formed in the lifting sleeve plate, a telescopic sleeve rod is fixedly installed at the bottom end of the middle of the lifting sleeve plate, and the conical punching head is fixedly installed at the bottom end of the telescopic sleeve rod. According to the utility model,the conical punching head is pushed to periodically move downwards through the rotation of the rotating disc, so that the punching action is realized, the punching intervals are equal, the punching period of the conical punching head can be adjusted, and the use is flexible.