The utility model belongs to the field of tobacco test devices, and particularly relates to a tobacco sample rolling and packing device. The device comprises a strip-shaped shaping plate, a notch mechanism and a buckle mechanism are arranged at the two ends of the shaping plate in the length direction correspondingly. The notch mechanism comprises a first notch and a second notch which extend in the length direction of the shaping plate and are symmetrical relative to the width center line of the shaping plate. A plurality of clamping notches are formed in the opposite sides of the first notchand the second notch at equal intervals in a perpendicular extending mode respectively, the clamping notches of the first notch and the second notch are correspondingly formed, the buckling mechanismcomprises two clamping blocks which are symmetrical relative to the width center line of the shaping plate and are perpendicular to the plate face of the shaping plate, and the clamping blocks are matched with the clamping notches. The tobacco leaf sample rolling device is simple in structure, capable of rapidly rolling multiple tobacco leaf samples and capable of being repeatedly used.