The utility model is suitable for the field of carriers. The utility model provides a turnover box for gas cylinders. The space in the turnover box is divided into a plurality of isolation areas through the supporting frame and the upper isolation assembly and the lower isolation assembly on the supporting frame. One gas cylinder can be placed in each isolation area, a plurality of gas cylinders can be turned over at a time, the isolation assembly is matched with the sponge groove in the bottom, the effect of temporarily fixing the gas cylinders is achieved, the gas cylinders are prevented from inclining or slipping or colliding, and operation is easy. In addition, the side face of the turnover box is made of metal, the discharging effect can be achieved, and static electricity is prevented from being generated and gathered.