The utility model relates to a light-emitting floor tile assembly structure which comprises a plurality of base section bars arranged on the ground, the base section bars are of a longitudinal long-strip structure, the lower portions of the base section bars are planes, and two longitudinal protruding strips are arranged on the upper portions of the base section bars. A mounting profile is connected to the upper part of the base profile, and is also of a longitudinal strip structure; the mounting sectional material is provided with a bottom transverse strip for placing the light-emitting plate; a longitudinal frame is vertically arranged on one side of the bottom transverse strip, the longitudinal frame extends towards the bottom transverse strip in the same direction to form a spacing strip used for containing the tempered glass panel, a groove matched with the longitudinal protruding strip is formed in the lower portion of the bottom transverse strip, and the installation sectional material is connected with the base sectional material through inserting connection of the groove and the protruding strip. An inserting or buckling structure is adopted for installation, the product is stably located on the groove clamping piece through the self weight of the product, and it is guaranteed that the product does not move up and down. Due to the fact that the concave grooves and theconvex grooves are buckled, left-right displacement is avoided, mounting and dismounting are very convenient, and the height of the product can be about 30 mm away from the ground.