The utility model discloses a poultry blood sampling auxiliary device which comprises a base provided with at least one operation face, the operation face and the bottom face of the base are arrangedin a 20-80-degree mode, and a label area easy to scrub is formed on the upper portion of the operation face in the horizontal direction. The at least one claw fixing device is arranged on the operation surface at intervals in the horizontal direction and located below the labeling area, and the claw fixing device comprises a clamping part used for fixing poultry claws. The poultry blood sampling device has the beneficial effects that the blood sampling work efficiency is improved, and the defective rate and the error rate of poultry caused in the blood sampling process are reduced.