A plant disease inoculation tool relates to the technical field of plant disease inoculation and comprises a fixing plate, a plurality of inoculation needles and an adjusting sheet. The fixing plate is provided with a plate surface. Wherein the plurality of inoculating needles are vertically fixed on the plate surface, needle tips of the plurality of inoculating needles extend towards the direction far away from the plate surface, the plurality of inoculating needles are arranged in an array of M rows and N columns, and M and N are positive integers greater than or equal to 2. Wherein the adjusting sheet is stacked on the plate surface, a plurality of through holes are formed in the adjusting sheet, the plurality of through holes and the plurality of inoculating needles are arranged in a one-to-one correspondence manner, and needle tips of the inoculating needles correspondingly penetrate through the corresponding through holes and extend out of the through holes. The plant disease inoculation tool can accurately control the stabbing degree of plant leaves and the distance between wounds in the plant disease inoculation process, and the plant disease inoculation effect and efficiency are remarkably improved.