The utility model discloses a multistage screening device for samples. The device comprises a bracket (2), wherein a cylindrical sample sieve (1) is hoisted on the bracket (2); wherein the sample sieve (1) is connected with a vibration device (4) arranged on the bracket (2); a sample receiving tray (5) for receiving and storing samples with different particle sizes is arranged below the sample sieve (1); a feeding hole is formed in the upper end of the sample sieve (1); a discharge hole is formed in the lower end of the sample sieve; more than one layer of screen (10) with screening aperturesfrom large to small is sequentially arranged in the sample screen (1) from top to bottom; an automatic sealing door (7) is arranged on the side wall of the sample sieve (1) at the same horizontal position as the upper surface of each layer of sieve (10); the sample sieve (1) is provided with a rotating device (3) used for throwing screened samples out of the automatic sealing doors (7), each automatic sealing door (7) is provided with a material guiding device (6) used for guiding the samples into the sample receiving disc (5), and the sample multistage screening device can separate the samples with different particle sizes, is convenient to collect, can save time and labor, and effectively improves efficiency.