The utility model discloses a packer grouting layered water stop simulation device. The device comprises a simulated drill hole, a simulated well casing, a filler and a simulated packing grouting device, the lower end of the simulation drill hole is closed; the lower end of the simulation well pipe is inserted through the upper end opening of the simulation drill hole; a filling space is reservedbetween the simulation well casing and the bottom wall of the simulation drill hole; the upper end of the simulation well pipe is positioned outside the upper end opening of the simulation drill hole;an annular space is reserved between the simulation well casing and the simulation drill hole; a water outlet is formed in the side wall of the lower end of the simulated drill hole; water outlet connecting transparent pipe, the simulation well pipe comprises a solid pipe section, a water filtering pipe section and a grouting window section. The simulated packing grouting device comprises a grouting pump, a grouting guide pipe, an upper packer, a grouting pipe, a lower packer and a high-pressure inflation device. The high-pressure inflation device is communicated with inflation rubber cylinders of the upper packer and the lower packer, a bottom plug is arranged at the bottom of a lower end cover of the lower packer in a sealed mode, the upper packer and the lower packer correspond to solid pipe sections adjacent to the grouting window respectively, and the grouting pipe corresponds to the grouting window section.