发明公开
EP0073554A1 Treatment of whole blood for determination of red blood cell volumes
失效
Behindlung von Vollblutfürdie Bestimmung der Volumina von rotenBlutkörperchen。
- 专利标题： Treatment of whole blood for determination of red blood cell volumes
- 专利标题（中）： Behindlung von Vollblutfürdie Bestimmung der Volumina von rotenBlutkörperchen。
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申请号： EP82302524.2申请日： 1982-05-18
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公开(公告)号： EP0073554A1公开(公告)日： 1983-03-09
- 发明人: Ornstein, Leonard , Kim, Young Ran
- 申请人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation)
- 申请人地址： 511 Benedict Avenue Tarrytown New York 10591-5097 US
- 专利权人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation)
- 当前专利权人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation)
- 当前专利权人地址： 511 Benedict Avenue Tarrytown New York 10591-5097 US
- 代理机构： Wain, Christopher Paul
- 优先权： US277539 19810626
- 主分类号： G01N33/50
- IPC分类号： G01N33/50 ; G01N33/48
摘要：
Whole blood is treated to sphere, and optionally also fix, red blood cells in order to be able better to determine their volume and (optionally) number, by treating the blood with (a) an isotonic solution of a sphering agent, and (b) an isotonic solution of a sphering agent and a protein, to provide a weight ratio of protein to sphering agent of from 20:1 to 70:1, and a final concentration of sphering agent of from 2 to 10 mg/100 ml. In a modification of this procedure, use is made of the protein endogenous to the blood and step (b) is replaced by (c) treating the mixture from step (a) with an isotomic solution of a fixing agent such as glutaraldehyde. Reagent compositions comprising a sphering agent and a protein, for use in the above method, are also described.
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