发明公开
EP0084796A2 HLA typing method and cDNA probes used therein
失效
Verfahren zur Charakterisierung von HLA und die darin benutzten cDNS-Testmittel。
- 专利标题： HLA typing method and cDNA probes used therein
- 专利标题（中）： Verfahren zur Charakterisierung von HLA und die darin benutzten cDNS-Testmittel。
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申请号： EP83100175.5申请日： 1983-01-11
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公开(公告)号： EP0084796A2公开(公告)日： 1983-08-03
- 发明人: Erlich, Henry A.
- 申请人： CETUS CORPORATION
- 申请人地址： 1400 Fifty-Third Street Emeryville California 94608 US
- 专利权人： CETUS CORPORATION
- 当前专利权人： CETUS CORPORATION
- 当前专利权人地址： 1400 Fifty-Third Street Emeryville California 94608 US
- 代理机构： Wuesthoff, Franz, Dr.-Ing.
- 优先权： US341902 19820122
- 主分类号： C12N15/00
- IPC分类号： C12N15/00 ; C12Q1/68
摘要：
HLA typing based on restriction length polymorphism is carried out by: digesting an individual's HLA DNA with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; subjecting the digest to genomic blotting using a labeled cDNA hybridization probe that is complementary to an HLA DNA sequence involved in the polymorphism; and comparing the resulting genomic blotting pattern with a standard This technique may be adapted to make paternity or transplant or transfusion compatibility determinations or to make disease association correlations to diagnose diseases or predict susceptibility to diseases. Locus specific cDNA hybridization probes, particularly probes for genes of Class II loci (D and OR loci), for use in the typing procedure are described.
公开/授权文献
- EP0084796B1 HLA typing method and cDNA probes used therein 公开/授权日：1990-05-02
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