发明公开
EP0096586A2 Temperature sensitive multicopy plasmids and organisms containing the same, their production and use in protein production 失效
包含相同和生物体,它们的制备和使用用于蛋白质生产热敏多拷贝质粒。

Temperature sensitive multicopy plasmids and organisms containing the same, their production and use in protein production
摘要:
The invention provides plasmids of ColEl type parentage which have a temperature sensitive replication system such that the plasmid copy number in a given host cell may be controlled by a temperature shift. Such plasmids permit the use of temperature as a switch for controlling gene amplification and hence the production of corresponding protein in a host cell.
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