用于多价抗原的测定三种不同的受体，其中所述第一和第三的存在于液相中并是可绑定与抗原，所述第二受体存在于固相和是可绑定与所述第一受体和所述第三受体携带标签 并且不跨越与第一和第二受体发生反应，所述抗原同时孵育或者与所有三种受体或者与第一和第二受体，然后分离各相，孵育，如果合适的话，与所述第三受体的固相，进行相再次分离，并且测量该标记 在阶段之一。

For the determination of a polyvalent antigen using three different receptors of which the first and the third are present in liquid phase and are bindable with the antigen, the second receptor is present in solid phase and to the first receptor is capable of binding and the third receptor carries a labeling and with the first and the second receptor is not cross-react, the antigen simultaneously incubated either with all three receptors or with the first and second receptor, then separates the phases, incubated, if appropriate, the solid phase with the third receptor, separating again the phases and measures the labeling in one of the phases.