发明公开
- 专利标题： Method of isolating restriction fragment deletions in Vibrio cholerae, and products thereof
- 专利标题（中）： 隔离胆汁中限制性片段残留的方法及其制备方法
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申请号： EP84301278申请日： 1984-02-28
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公开(公告)号： EP0119031A3公开(公告)日： 1985-09-18
- 发明人: Kaper, James B. , Levine, Myron M.
- 申请人： THE UNIVERSITY OF MARYLAND AT BALTIMORE
- 专利权人： THE UNIVERSITY OF MARYLAND AT BALTIMORE
- 当前专利权人： THE UNIVERSITY OF MARYLAND AT BALTIMORE
- 优先权： US472276 19830304; US581406 19840217
- 主分类号： C12N15/00
- IPC分类号： C12N15/00 ; C12N01/20 ; A61K39/106 ; C12R01/63
摘要：
This invention relates to a method of isolating deletion mutants of Vibrio cholerae ,wherein the deletion is predetermined by digestion with restriction endonucleases of known specificity. The deletions are inserted into the Vibrio cholerae chromosone by in vivo recombination between a plasmid carrying the desired deletion, with adjacent flanking sequences, and the Vibrio cholerae chromosome. The invention includes the isolation and characterization of a new Vibrio cholerae strain having a deletion in the tox gene, as defined by Acc I, Xba I, C/a I and/or restriction endonuclease sites.
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