New compounds, 2',3'-dideoxy-2'-fluorokanamycin A and 1-N-(a-hydroxy-w-aminoalkanoyl) derivatives thereof, particularly 1-N-(DL- or L-3-amino-2-hydroxypropionyl)- and 1-N-(L-4-amino-2-hydroxybutyryt)-2',3'-dideoxy-2'-fluorokanamycins A are now provided, which are each useful as anti-bacterial agent. 2',3'-Dideoxy-2'-fluorokanamycin A is prepared by a process comprising condensing a 6-azido-4-0-protected-2,3-6-trideoxy-2-fluoro-a-D-ribo-hexopyranosyl bromide with the 4-hydroxyl group of a 6-0-(2',4',6'-tri-0- protected-3'-N-protected-3'-amino-3'-deoxy-α-D-glucopyranosyl)-1,3-bis-N-protected-2-deoxystreptamine, reducing the resulting condensation product to convert its azido group into an amino group, and removing the remaining amino-protecting and hydroxyl-protecting groups from the reduction product.