发明公开
- 专利标题： Purified deacetoxycephalosporin C synthase
- 专利标题（中）： Gereinigte脱乙酰氧头孢菌素-C合酶。
-
申请号： EP89310744.1申请日： 1989-10-19
-
公开(公告)号： EP0366354A2公开(公告)日： 1990-05-02
- 发明人: Dotzlaf, Joe Edward , Yeh, Wu-Kuang
- 申请人： ELI LILLY AND COMPANY
- 申请人地址： Lilly Corporate Center Indianapolis, Indiana 46285 US
- 专利权人： ELI LILLY AND COMPANY
- 当前专利权人： ELI LILLY AND COMPANY
- 当前专利权人地址： Lilly Corporate Center Indianapolis, Indiana 46285 US
- 代理机构： Tapping, Kenneth George
- 优先权： US261437 19881024
- 主分类号： C12N9/00
- IPC分类号： C12N9/00 ; C12P35/06
摘要：
Deacetoxycephalosporin C synthase obtained from cell-free extracts of Streptomyces clavuligerus was purified to near homogeneity and characterized in inactive form. The amino acid sequence was determined and the synthase recombinantly reproduced in active form in E. coli . The recombinantly produced synthase is provided in about 97% pure form by a chromatographic process. The synthase lacks DAOC hydroxylase function; however, it possesses the ability to transform 3-exomethylenecephalosporin C to deacetylcephalosporin C.
公开/授权文献
- EP0366354B1 Purified deacetoxycephalosporin C synthase 公开/授权日：1996-01-03
信息查询
