发明公开
- 专利标题： Cholesterol oxidase
- 专利标题（中）： Cholesterinoxidase。
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申请号： EP91303164.7申请日： 1991-04-10
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公开(公告)号： EP0452112A1公开(公告)日： 1991-10-16
- 发明人: Fujishiro , Kinya , Uwajima, Takayuki
- 申请人： KYOWA HAKKO KOGYO CO., LTD.
- 申请人地址： 6-1, Ohte-Machi Itchome Chiyoda-ku, Tokyo JP
- 专利权人： KYOWA HAKKO KOGYO CO., LTD.
- 当前专利权人： KYOWA HAKKO KOGYO CO., LTD.
- 当前专利权人地址： 6-1, Ohte-Machi Itchome Chiyoda-ku, Tokyo JP
- 代理机构： Lambert, Hugh Richmond
- 优先权： JP94296/90 19900410
- 主分类号： C12N15/53
- IPC分类号： C12N15/53 ; C12N9/04 ; C12Q1/26 ; C12Q1/60
摘要：
A novel cholesterol oxidase is disclosed having a high substrate affinity, high activity and operable over a wide pH range, including acid value. The enzyme is produced in low yield by microorganisms of the species Brevibacterium sterolicum . Improved yields are obtained by transforming microorganisms of the species E.coli with the DNA from Brevibacterium sterolicum and which encodes the novel enzyme.
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