发明公开
EP0490286A1 Enzymatic composition for ethanol assay
失效
Enzymatische zusammensetzung zur Bestimmung vonÄthanol。
- 专利标题： Enzymatic composition for ethanol assay
- 专利标题（中）： Enzymatische zusammensetzung zur Bestimmung vonÄthanol。
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申请号： EP91120950.0申请日： 1991-12-06
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公开(公告)号： EP0490286A1公开(公告)日： 1992-06-17
- 发明人: Kaufman, Richard Allen , Rosenfeld, Henry Joseph
- 申请人： F. HOFFMANN-LA ROCHE AG
- 申请人地址： Postfach 3255 CH-4002 Basel CH
- 专利权人： F. HOFFMANN-LA ROCHE AG
- 当前专利权人： F. HOFFMANN-LA ROCHE AG
- 当前专利权人地址： Postfach 3255 CH-4002 Basel CH
- 代理机构： Notegen, Eric-André
- 优先权： US628080 19901213
- 主分类号： C12Q1/32
- IPC分类号： C12Q1/32 ; C12N9/96
摘要：
An enzymatic assay composition for ethanol determination is provided comprising
(a) enzyme alcohol dehydrogenase;
(b) coenzyme nicotinamide adenine dinucleotide; and
(c) a compound of the formula
H₂N-(CH₂) n -CHR-CH₂-NH₂ I
where R is H, CH₃, or OH; and n is 0, 1, 2 , 3 or 4 with the proviso that when R is OH, n cannot be 0.
Compound I acts as a buffer and a trapping agent for a product of the enzymatic reaction. Long stability of the assay composition is achieved.
(a) enzyme alcohol dehydrogenase;
(b) coenzyme nicotinamide adenine dinucleotide; and
(c) a compound of the formula
H₂N-(CH₂) n -CHR-CH₂-NH₂ I
where R is H, CH₃, or OH; and n is 0, 1, 2 , 3 or 4 with the proviso that when R is OH, n cannot be 0.
Compound I acts as a buffer and a trapping agent for a product of the enzymatic reaction. Long stability of the assay composition is achieved.
公开/授权文献
- EP0490286B1 Enzymatic composition for ethanol assay 公开/授权日：1995-08-02
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