发明授权
EP0589877B1 METHODS FOR PRODUCING FUNCTIONAL, SINGLE-CHAIN Fv ANTIBODY FRAGMENTS ON THE SURFACE OF BACTERIOPHAGE PARTICLES
失效
METHOD FOR产生功能性,单链Fv抗体片段噬菌体ON SURFACES
- 专利标题: METHODS FOR PRODUCING FUNCTIONAL, SINGLE-CHAIN Fv ANTIBODY FRAGMENTS ON THE SURFACE OF BACTERIOPHAGE PARTICLES
- 专利标题(中): METHOD FOR产生功能性,单链Fv抗体片段噬菌体ON SURFACES
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申请号: EP91913039.3申请日: 1991-07-10
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公开(公告)号: EP0589877B1公开(公告)日: 1996-11-13
- 发明人: McCAFFERTY, John , POPE, Anthony, Richard , JOHNSON, Kevin Stuart , HOOGENBOOM, Hendricus Renerus Jacobus Mattheus , GRIFFITHS, Andrew David , JACKSON, Ronald, Henry , HOLLIGER, Kaspar, Philipp , MARKS, James David , CLACKSON, Timothy, Piers , CHISWELL, David John , WINTER, Gregory, Paul , BONNERT, Timothy, Peter
- 申请人: CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , MEDICAL RESEARCH COUNCIL
- 申请人地址: The Science Park Melbourn, Cambridgeshire SG8 6EJ GB
- 专利权人: CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED,MEDICAL RESEARCH COUNCIL
- 当前专利权人: CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED,MEDICAL RESEARCH COUNCIL
- 当前专利权人地址: The Science Park Melbourn, Cambridgeshire SG8 6EJ GB
- 代理机构: Paget, Hugh Charles Edward
- 优先权: GB9015198 19900710; GB9022845 19901019; GB9024503 19901112; GB9104744 19910306; GB9110549 19910515
- 国际公布: WO9201047 19920123
- 主分类号: C12N7/01
- IPC分类号: C12N7/01 ; C07K1/04 ; G01N33/531
摘要:
A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
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