发明公开
EP0649472A1 FUSION PROTEINS COMPRISING EUKARYOTIC CYTOCHROME P450 FUSED TO A REDUCTASE
失效
融合蛋白的耦合到A还原酶真核细胞色素P450。
- 专利标题: FUSION PROTEINS COMPRISING EUKARYOTIC CYTOCHROME P450 FUSED TO A REDUCTASE
- 专利标题(中): 融合蛋白的耦合到A还原酶真核细胞色素P450。
-
申请号: EP93916837.0申请日: 1993-06-29
-
公开(公告)号: EP0649472A1公开(公告)日: 1995-04-26
- 发明人: FISHER, Charles, W. , BARNES, Henry, J. , ESTABROOK, Ronald, W.
- 申请人: BOARD OF REGENTS THE UNIVERSITY OF TEXAS SYSTEM
- 申请人地址: 201 West 7th Street Austin, Texas 78701 US
- 专利权人: BOARD OF REGENTS THE UNIVERSITY OF TEXAS SYSTEM
- 当前专利权人: BOARD OF REGENTS THE UNIVERSITY OF TEXAS SYSTEM
- 当前专利权人地址: 201 West 7th Street Austin, Texas 78701 US
- 代理机构: Grünecker, Kinkeldey, Stockmair & Schwanhäusser Anwaltssozietät
- 优先权: US19920908317 19920702
- 国际公布: WO1994001568 19940120
- 主分类号: C12N1
- IPC分类号: C12N1 ; C12N9 ; C12N15
摘要:
Disclosed are methods and compositions for effecting bacterial expression of eukaryotic cytochrome P450 enzymes and fusion proteins comprising a eukaryotic P450 domain fused to a reductase enzyme domain in a biologically active form. Certain embodiments involve the expression of eukaryotic cytochrome P45017α- hydroxylase which is expressed in large amounts in an E. coli host in a biologically active form without the need for coexpression or admixture of a cytochrome P450 reductase. Methods and compositions are also disclosed for the construction of cytochrome P450 enzyme hybrids or fusion proteins, incorporating the N-terminal 9 amino acids from bovine 17α-hydroxylase, which will enable many eukaryotic cytochrome P450 enzymes or fusion proteins to be expressed in bacteria.
信息查询