发明公开
EP0649472A1 FUSION PROTEINS COMPRISING EUKARYOTIC CYTOCHROME P450 FUSED TO A REDUCTASE 失效
融合蛋白的耦合到A还原酶真核细胞色素P450。

FUSION PROTEINS COMPRISING EUKARYOTIC CYTOCHROME P450 FUSED TO A REDUCTASE
摘要:
Disclosed are methods and compositions for effecting bacterial expression of eukaryotic cytochrome P450 enzymes and fusion proteins comprising a eukaryotic P450 domain fused to a reductase enzyme domain in a biologically active form. Certain embodiments involve the expression of eukaryotic cytochrome P45017α- hydroxylase which is expressed in large amounts in an E. coli host in a biologically active form without the need for coexpression or admixture of a cytochrome P450 reductase. Methods and compositions are also disclosed for the construction of cytochrome P450 enzyme hybrids or fusion proteins, incorporating the N-terminal 9 amino acids from bovine 17α-hydroxylase, which will enable many eukaryotic cytochrome P450 enzymes or fusion proteins to be expressed in bacteria.
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