An enzyme preparation comprising a modified enzyme selected from the group consisting of an amylase, lipase, oxidoreductase, pectinase or hemicellulase, the modified enzyme having an improved performance due to an alkaline pI and/or increased surface activity obtained by chemical modification or amino acid substitution, is useful e.g. in detergents, in baking flour, in animal feed, in the manufacture of cellulosic fabrics and for the treatment of lignocellulosic fibers.