发明公开
- 专利标题: OPTICAL SPECIMEN ANALYSIS SYSTEM AND METHOD
- 专利标题(中): 光测试分析系统和方法
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申请号: EP94909763.0申请日: 1994-02-24
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公开(公告)号: EP0731951A1公开(公告)日: 1996-09-18
- 发明人: CHU, Albert, E. , TAO, Lian
- 申请人: E-Y LABORATORIES, INC.
- 申请人地址: 107 North Amphlett Boulevard San Mateo California 94401 US
- 专利权人: E-Y LABORATORIES, INC.
- 当前专利权人: E-Y LABORATORIES, INC.
- 当前专利权人地址: 107 North Amphlett Boulevard San Mateo California 94401 US
- 代理机构: Cross, Rupert Edward Blount, et al
- 优先权: US19930023113 19930226
- 国际公布: WO1994019767 19940901
- 主分类号: G01N21
- IPC分类号: G01N21 ; G06T7 ; G01N15
摘要:
A method and system for automatically analyzing the presence of an analyte in a liquid specimen of biological origin are described. The liquid specimen is added to a testing substrate that has a receptor immobilized on a portion of its surface. If analyte is present in the specimen, it will specifically bind to the receptor. A labeled reagent is added to the testing substrate which will bind to the analyte, if present, and generate a color on the testing substrate. The testing substrate is then illuminated and, using electronic equipment, a digital image of its surface is acquired and automatically scanned to locate an area that has the highest color density, which corresponds to the presence of labeled reagent. A measurement of color density, corresponding to pixels per unit area, is generated. An area peripheral to the area of highest color density, which represents background density, is also located and a measured. The presence or absence of analyte in the liquid sample is determined by adjusting the measurement of labeled reagent with the background density measurement in accordance with a predefined mathematical function. In some cases, the result of the test will be interpreted as a binary, positive/negative result according to whether the measurement taken is above or below a given threshold. In other cases, a continuous range of values may be generated from different samples that correspond to the concentration of the analyte present in each tested sample.
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