发明授权
EP0841942B1 PROCEDE DE PRODUCTION INDUSTRIELLE D'UN VACCIN CONTRE L'ENCEPHALITE JAPONAISE ET VACCIN OBTENU 失效
法的日本脑炎疫苗,河北工业生产,所以得了疫苗

  • 专利标题: PROCEDE DE PRODUCTION INDUSTRIELLE D'UN VACCIN CONTRE L'ENCEPHALITE JAPONAISE ET VACCIN OBTENU
  • 专利标题(英): Method for industrially producing a japanese encephalitis vaccine, and resulting vaccine
  • 专利标题(中): 法的日本脑炎疫苗,河北工业生产,所以得了疫苗
  • 申请号: EP96927102.2
    申请日: 1996-07-29
  • 公开(公告)号: EP0841942B1
    公开(公告)日: 2003-01-29
  • 发明人: FANGET, BernardFRANCON, AlainHEIMENDINGER, Pierre
  • 申请人: Aventis Pasteur
  • 申请人地址: 2, Avenue pont Pasteur 69367 Lyon Cedex 07 FR
  • 专利权人: Aventis Pasteur
  • 当前专利权人: Aventis Pasteur
  • 当前专利权人地址: 2, Avenue pont Pasteur 69367 Lyon Cedex 07 FR
  • 代理机构: Bernasconi, Jean
  • 优先权: FR9509374 19950801; FR9603638 19960322
  • 国际公布: WO97004803 19970213
  • 主分类号: A61K39/12
  • IPC分类号: A61K39/12 C12N7/02
PROCEDE DE PRODUCTION INDUSTRIELLE D'UN VACCIN CONTRE L'ENCEPHALITE JAPONAISE ET VACCIN OBTENU
摘要:
A method is disclosed for industrially producing a Japanese encephalitis vaccine, wherein (a) cells from a cell line are cultured, (b) the resulting cell culture is inoculated with a Japanese encephalitis virus in the presence of a viral growth medium, (c) the virus is propagated and multiplied on the cells, (d) the viral growth medium is recovered in the form of a suspension of viruses produced by the cells, (e) the virus suspension is purified in at least one ion exchange chromatography step and a gel permeation step, and (f) the virus suspension is formulated and converted into a pharmaceutical form to preserve it until the moment of use. A Japanese encephalitis vaccine characterised in that it comprises a Japanese encephalitis virus produced by culturing cells from a cell line, and in that the cellular DNA content is less than 100 pg/dose, is also disclosed.
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