发明授权
- 专利标题: PROCEDE DE PRODUCTION INDUSTRIELLE D'UN VACCIN CONTRE L'ENCEPHALITE JAPONAISE ET VACCIN OBTENU
- 专利标题(英): Method for industrially producing a japanese encephalitis vaccine, and resulting vaccine
- 专利标题(中): 法的日本脑炎疫苗,河北工业生产,所以得了疫苗
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申请号: EP96927102.2申请日: 1996-07-29
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公开(公告)号: EP0841942B1公开(公告)日: 2003-01-29
- 发明人: FANGET, Bernard , FRANCON, Alain , HEIMENDINGER, Pierre
- 申请人: Aventis Pasteur
- 申请人地址: 2, Avenue pont Pasteur 69367 Lyon Cedex 07 FR
- 专利权人: Aventis Pasteur
- 当前专利权人: Aventis Pasteur
- 当前专利权人地址: 2, Avenue pont Pasteur 69367 Lyon Cedex 07 FR
- 代理机构: Bernasconi, Jean
- 优先权: FR9509374 19950801; FR9603638 19960322
- 国际公布: WO97004803 19970213
- 主分类号: A61K39/12
- IPC分类号: A61K39/12 ; C12N7/02
摘要:
A method is disclosed for industrially producing a Japanese encephalitis vaccine, wherein (a) cells from a cell line are cultured, (b) the resulting cell culture is inoculated with a Japanese encephalitis virus in the presence of a viral growth medium, (c) the virus is propagated and multiplied on the cells, (d) the viral growth medium is recovered in the form of a suspension of viruses produced by the cells, (e) the virus suspension is purified in at least one ion exchange chromatography step and a gel permeation step, and (f) the virus suspension is formulated and converted into a pharmaceutical form to preserve it until the moment of use. A Japanese encephalitis vaccine characterised in that it comprises a Japanese encephalitis virus produced by culturing cells from a cell line, and in that the cellular DNA content is less than 100 pg/dose, is also disclosed.
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