发明公开
- 专利标题： EMBRYONIC STEM CELL SERUM REPLACEMENT
- 专利标题（中）： 代换胚胎干细胞小生境血清
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申请号： EP98901221.6申请日： 1998-01-09
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公开(公告)号： EP0986635A1公开(公告)日： 2000-03-22
- 发明人: PRICE, Paul, J. , GOLDSBOROUGH, Mindy, D. , TILKINS, Mary, Lynn
- 申请人： Life Technologies, Inc.
- 申请人地址： 9800 Medical Center Drive Rockville, MD 20850 US
- 专利权人： Life Technologies, Inc.
- 当前专利权人： Life Technologies, Inc.
- 当前专利权人地址： 9800 Medical Center Drive Rockville, MD 20850 US
- 代理机构： Ritter, Stephen David
- 优先权： US781772 19970110
- 国际公布： WO9830679 19980716
- 主分类号： C12N5/00
- IPC分类号： C12N5/00 ; C12N5/02 ; C12N5/06 ; C12N5/08 ; C12N5/10 ; C12N15/00 ; C12N1/38
摘要：
The present invention provides a serum-free supplement which supports the growth of embryonic stem cells in culture. Also provided are a medium comprising a basal medium supplemented with the serum-free supplement of the present invention. The present invention also provides methods for culturing and isolating embryonic stem cells, methods for producing a transgenic animal, and methods for expressing recombinant protein in embryonic stem cells and transgenic animals.
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