发明公开
- 专利标题： HIGH FIDELITY POLYMERASES AND USES THEREOF
- 专利标题（中）： 高灵敏度聚合酶和用途
-
申请号： EP98943434.5申请日： 1998-08-28
-
公开(公告)号： EP1012161A1公开(公告)日： 2000-06-28
- 发明人: YANG, Shuwei , CHATTERJEE, Deb, K.
- 申请人： LIFE TECHNOLOGIES, INC.
- 申请人地址： 9800 Medical Center Drive,P.O. Box 6482 Rockville, MD 20850 US
- 专利权人： LIFE TECHNOLOGIES, INC.
- 当前专利权人： LIFE TECHNOLOGIES, INC.
- 当前专利权人地址： 9800 Medical Center Drive,P.O. Box 6482 Rockville, MD 20850 US
- 代理机构： Schlich, George William
- 优先权： US56263P 19970829; US60131P 19970926; US85247P 19980513; US141522 19980827
- 国际公布： WO9910366 19990304
- 主分类号： C07H21/04
- IPC分类号： C07H21/04 ; C12N9/12 ; C12P19/34
摘要：
The present invention relates to a DNA and RNA polymerases which have increased fidelity (or reduced misincorporation rate). In particular, the invention relates to a method of making such polymerases by modifying or mutating the nucleotide binding domain of the polymerase (e.g., the O-helix). The invention also relates to DNA molecules containing the genes encoding the polymerases of the invention, to host cells containing such DNA molecules and to methods to make the polymerases using the host cells. The polymerases are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.
公开/授权文献
- EP1012161B1 HIGH FIDELITY POLYMERASES AND USES THEREOF 公开/授权日：2011-03-16
信息查询
