发明授权
- 专利标题: SYNTHETIC PROMOTERS
- 专利标题(中): 合成启动
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申请号: EP99908362.9申请日: 1999-02-23
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公开(公告)号: EP1056875B1公开(公告)日: 2007-08-29
- 发明人: BOWEN, Benjamin, A. , BRUCE, Wesley, B. , LU, Guihua , SIMS, Lynne, E. , TAGLIANI, Laura, A.
- 申请人: PIONEER HI-BRED INTERNATIONAL, INC.
- 申请人地址: 800 Capital Square, 400 Locust Street Des Moines, Iowa 50309 US
- 专利权人: PIONEER HI-BRED INTERNATIONAL, INC.
- 当前专利权人: PIONEER HI-BRED INTERNATIONAL, INC.
- 当前专利权人地址: 800 Capital Square, 400 Locust Street Des Moines, Iowa 50309 US
- 代理机构: Bentham, Andrew
- 优先权: US28819 19980224
- 国际公布: WO1999043838 19990902
- 主分类号: C12N15/82
- IPC分类号: C12N15/82 ; C12N5/10 ; C12N9/02 ; A01H5/00
摘要:
Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a 'TATA to start' sequence containing 64 % or greater GC content and a synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.
公开/授权文献
- EP1056875A1 SYNTHETIC PROMOTERS 公开/授权日:2000-12-06
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