Techniques for regulating membrane denaturation reactions without resort to any physical shear force. Namely, a method which comprises reacting a stimulus (light, etc.) with a compound which is activated by this stimulus on a membrane such as a biomembrane to thereby induce membrane destruction at a specific site; and membrane structures (cells, etc.) with membrane destruction at a specific site obtained thereby. Use of these membrane structures makes it possible to transfer genes, etc. Materials for causing membrane destruction at a specific site. Use of these techniques makes it possible to easily pierce cell membranes with ultrafine members constituting, for example, microinjectors, micromanipulators or microelectrodes which are hardly usable in piercing cell membranes by the conventional techniques.