发明公开
EP1134284A1 CREATINE AMIDINOHYDROLASE AND PROCESS FOR PRODUCING THE SAME
审中-公开
KRERTIN-AMIDOHYDROLASE UND VERFAHREN ZU DEREN HERSTELLUNG
- 专利标题： CREATINE AMIDINOHYDROLASE AND PROCESS FOR PRODUCING THE SAME
- 专利标题（中）： KRERTIN-AMIDOHYDROLASE UND VERFAHREN ZU DEREN HERSTELLUNG
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申请号： EP99972676.3申请日： 1999-11-25
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公开(公告)号： EP1134284A1公开(公告)日： 2001-09-19
- 发明人: FURUKAWA, Keisuke , ICHIKAWA, Toshio
- 申请人： KIKKOMAN CORPORATION
- 申请人地址： 250 Noda Noda-shi, Chiba 278-8601 JP
- 专利权人： KIKKOMAN CORPORATION
- 当前专利权人： KIKKOMAN CORPORATION
- 当前专利权人地址： 250 Noda Noda-shi, Chiba 278-8601 JP
- 代理机构： Brasnett, Adrian Hugh
- 优先权： JP33425298 19981125
- 国际公布： WO0031245 20000602
- 主分类号： C12N9/78
- IPC分类号： C12N9/78
摘要：
The present invention relates to a creatine amidinohydrolase having the following physicochemical properties: (a) hydrolyzing 1 mole of creatine to generate 1 mole of sarcosine and 1 mole of urea, (b) having a substrate specificity to creatine, (c) having an optimum pH ranging from 6.0 to 7.0, particularly a pH of about 6.5, (d) having a stable pH ranging from 4.0 to 11.0, (e) having an optimum temperature ranging from 50 to 55°C, and (f) having a molecular weight of approximately 92,000 daltons (as measured by gel filtration); and to a method for producing a creatine amidinohydrolase, which comprises, culturing a microorganism having an ability to produce the creatine amidinohydrolase, and recovering the creatine amidinohydrolase from the obtained culture. The creatine amidinohydrolase of the invention is characterized by being insusceptible to bilirubin when measuring creatinine, since its optimum pH is in the weakly acidic range.
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