A virus-producing cell sustaining an ability to produce a virus at a high titer is successfully constructed by expressing a virus structural gene under the regulation by EF1α promoter. In this virus-producing cell, the virus structural gene is ligated to a selection marker gene via IRES and domains other than the protein coding domain are eliminated from the DNA encoding the virus structural protein. Thus, reduction of the titer due to the cell passage can be prevented and the appearance of a wild type virus caused by unfavorable recombination of the virus genome can be inhibited.