发明公开
- 专利标题： DIRECTED EVOLUTION OF OXIDASE ENZYMES
- 专利标题（中）： 氧化酶酶寻找EVOLUTION
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申请号： EP00980811.4申请日： 2000-11-27
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公开(公告)号： EP1290147A1公开(公告)日： 2003-03-12
- 发明人: ARNOLD, Frances, H. , PETROUNIA, Ioanna P. , SUN, Lianhong
- 申请人： California Institute of Technology , Arnold, Frances , Petrounia, Ioanna P. , Sun, Lianhong
- 申请人地址： 1200 East California Boulevard,Mail Code 201-85 Pasadena, CA 91125 US
- 专利权人： California Institute of Technology,Arnold, Frances,Petrounia, Ioanna P.,Sun, Lianhong
- 当前专利权人： California Institute of Technology,Arnold, Frances,Petrounia, Ioanna P.,Sun, Lianhong
- 当前专利权人地址： 1200 East California Boulevard,Mail Code 201-85 Pasadena, CA 91125 US
- 代理机构： Howard, Paul Nicholas
- 优先权： US571553 20000516
- 国际公布： WO01088110 20011122
- 主分类号： C12N9/00
- IPC分类号： C12N9/00 ; C12N15/00 ; C07H21/04
摘要：
This invention relates to the expression of improved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly oxidase enzymes. The enzymes are advantageously produced in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved expression, enhanced activity toward one or more substrates, and increased thermal stability. In a particular embodiment, the invention relates to improved expression of the galactose oxidase gene and galactose oxidase enzymes. GAO mutants that are highly active and/or thermostable are disclosed.
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